LOCUS       pLV-[hsa-miR-371_372_373]   10143 bp    DNA     circular     12-APR-2007
SOURCE      
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     misc_feature    1807..1929
                     /label=cPPT
     CDS             8064..8924
                     /label=Amp(R)
     promoter        9125..9665
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             6669..6903
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(7478..7933)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    6975..7105
                     /label=SV40 pA
     misc_feature    7106..7319
                     /label=SV40 ori
     misc_feature    5990..6586
                     /label=WPRE
     promoter        1933..2328
                     /label=human EF1a promoter
     intron          2361..4608
                     /label=intron
     CDS             4655..5980
                     /label=rPuro (RFP Puro)
     misc_RNA        3105..4403
                     /label=hsa-mir-371_372_373 precursor
BASE COUNT     2387 a      2624 c      2789 g      2343 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgagagctca tcaagggcta 
     3121 ctctccacct ccttgcttaa aagcctcttc tgatgggtaa gtgcttccac ttgcgatcgc 
     3181 cgccttgccg catcccctca gcctgtggca ctcaaactgt gggggcactt tctgctctct 
     3241 ggtgaaagtg ccgccatctt ttgagtgtta ccgcttgaga agactcaacc tgcggagaag 
     3301 ataccatttt gattgggtga gggggcgggt agcaggatgg ccctagaccc tgcctatggc 
     3361 cgtttcctcg tgatataaat ttcttggccg gggctcttgc agatggagct gctcaccctg 
     3421 tgggcctcaa atgtggagca ctattctgat gtccaagtgg aaagtgctgc gacatttgag 
     3481 cgtcaccggt gacgcccata tcaacggatg ccgtggagct cggtcttctg caggaactaa 
     3541 agagcctgtg gtttcgattc ccagccggaa actgtcttgg gtacaggtcc cttacagcgt 
     3601 ctggctgtaa tggctccgga aaacctgggg aagggaagag ggcctcctgg gctctcacct 
     3661 gacacaacta agggaatctg ggttagctgg tgaaaggaag agaaggttca gagggcggct 
     3721 gtgcgcctgc gccgggcgcg gcggctcaca cctgtaatcc cagcacgttg ggaggccgag 
     3781 gcaggcggat cacgaggtca ggagatcgag accatcctgg ctaacacggt gaaaccccgt 
     3841 ctctactaaa aatacaaaaa atgagccggg cgtggtggcg ggcgcctgta gtcccagcta 
     3901 cttgggaggc tgaggcagga gaatggcgtg aacccgggag gcggagtttg cagtgagccg 
     3961 agatcgcgcc accgcactcc agcctgggcg acagagcaag actcattctc aaagaaaaaa 
     4021 aaaaaaaaaa acagcagctg tgaccaaggg gctgtatgca caaagaaagg aatgcttttt 
     4081 gctgttggtg ttataattga tatgtaactt tcctcttccc ccatagcaag caagtttatt 
     4141 gctgacattt aacatgtgct tggatgattg gaggccaacc tcttgtaggc aagaaagtca 
     4201 cagtgatggc agatcctcgc gaggagctca tactgggata ctcaaaatgg gggcgctttc 
     4261 ctttttgtct gtactgggaa gtgcttcgat tttggggtgt ccctgtttga gtagggcatc 
     4321 acgaaccatc ctgcttcaag ggagcctgcg ggtctgactg cagcttcagc tatgacctgg 
     4381 agttcccggg cttctctgcg gggctcgagc ttttggagta cgtcgtcttt aggttggggg 
     4441 gaggggtttt atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca 
     4501 gcttggcact tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc 
     4561 attctcaagc ctcagacagt ggttcaaagt ttttttcttc catttcaggt gtcgtgaaaa 
     4621 ctacccctct agagtcgagc taccggtcgc caccatggtg agcaagggcg aggaggataa 
     4681 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg 
     4741 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc 
     4801 caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca 
     4861 gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa 
     4921 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt 
     4981 ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct 
     5041 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga 
     5101 ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag 
     5161 gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa 
     5221 gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca 
     5281 caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg 
     5341 cggcatggac gagctgtaca aggacccacc ggtcgccacc atgaccgagt acaagcccac 
     5401 ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt 
     5461 cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac 
     5521 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc 
     5581 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt 
     5641 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca 
     5701 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac 
     5761 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg 
     5821 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa 
     5881 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagt gcccgaagga 
     5941 ccgcgcgacc tggtgcatga cccgcaagcc cggtgcctga gcggccgcgt cgacaatcaa 
     6001 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt 
     6061 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct 
     6121 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc 
     6181 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg 
     6241 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc 
     6301 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc 
     6361 actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt 
     6421 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca 
     6481 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt 
     6541 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat tcgagctcgg 
     6601 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa 
     6661 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta 
     6721 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc 
     6781 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt 
     6841 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta 
     6901 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat 
     6961 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca 
     7021 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac 
     7081 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc 
     7141 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat 
     7201 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt 
     7261 ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca 
     7321 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc 
     7381 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc 
     7441 ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta 
     7501 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg 
     7561 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa 
     7621 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc 
     7681 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt 
     7741 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca 
     7801 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc 
     7861 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta 
     7921 acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat 
     7981 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc 
     8041 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct 
     8101 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag 
     8161 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta 
     8221 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc 
     8281 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca 
     8341 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg 
     8401 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg 
     8461 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca 
     8521 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa 
     8581 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa 
     8641 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata 
     8701 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat 
     8761 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc 
     8821 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata 
     8881 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt 
     8941 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga 
     9001 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag 
     9061 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa 
     9121 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag 
     9181 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg 
     9241 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat 
     9301 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta 
     9361 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg 
     9421 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc 
     9481 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa 
     9541 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc 
     9601 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt 
     9661 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct 
     9721 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc 
     9781 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg 
     9841 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt 
     9901 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc 
     9961 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc 
    10021 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct 
    10081 atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc 
    10141 tgc 
//
